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TET2 antibody [GT649]

KO/KD
Anti-TET2 antibody [GT649] used in ChIP assay (ChIP assay). GTX629881
Anti-TET2 antibody [GT649] used in Immunoprecipitation (IP). GTX629881
Anti-TET2 antibody [GT649] used in Western Blot (WB). GTX629881
Anti-TET2 antibody [GT649] used in Western Blot (WB). GTX629881

Cat. No. GTX629881

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT649

Isotype

 IgG1

Application

 WB, IP, ChIP assay, EM

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TET2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 2 , KIAA1546 , MDS

Database

Research Area

DATA IMAGES

Anti-TET2 antibody [GT649] used in ChIP assay (ChIP assay). GTX629881

GTX629881 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
ChIP experiment and primer designs are based on Nat Commun. 2013;4:2166.

Anti-TET2 antibody [GT649] used in Immunoprecipitation (IP). GTX629881

GTX629881 IP Image

TET2 antibody [GT649] immunoprecipitates TET2 protein in IP experiments.
IP samples: 30 μg whole cell extract of TET2-transfected 293T cells.
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TET2 protein by 5 μg TET2 antibody [GT649] (GTX629881)
5 % SDS-PAGE
The immunoprecipitated TET2 protein was detected by TET2 antibody [GT649] (GTX629881) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-TET2 antibody [GT649] used in Western Blot (WB). GTX629881

GTX629881 WB Image

KO/KD

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [GT649] (GTX629881) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET2 antibody [GT649] used in Western Blot (WB). GTX629881

GTX629881 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [GT649] (GTX629881) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations