APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TFII-I. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
general transcription factor IIi , BAP135 , BTKAP1 , DIWS , GTFII-I , IB291 , SPIN , TFII-I , WBS , WBSCR6
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
Database
DATA IMAGES
![Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273](/templates/default/js/lightbox/images/loading.gif "Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273")
|
GTX637273 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX637273 WB Image
Various whole cell extracts (30 Âμg) were separated by 5% SDS-PAGE, and the membrane was blotted with TFII-I antibody [HL1674] (GTX637273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX637273 ICC/IF Image
TFII-I antibody [HL1674] detects TFII-I protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TFII-I stained by TFII-I antibody [HL1674] (GTX637273) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
|
REFERENCE
There are currently no references for TFII-I antibody [HL1674] (GTX637273). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TFII-I antibody [HL1674] (GTX637273). Be the first to share your experience with this product.
![Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273](/upload/website/prouct_img/small/GTX637273/GTX637273_T-44760_20221014_WB_shRNA_watermark_22101717_269.webp "Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273")
![Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273](/upload/website/prouct_img/small/GTX637273/GTX637273_T-44760_20220805_WB_22080923_205.webp "Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273")
![Anti-TFII-I antibody [HL1674] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637273](/upload/website/prouct_img/small/GTX637273/GTX637273_T-44760_20220916_ICC_IF_22102723_434.webp "Anti-TFII-I antibody [HL1674] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637273")
![Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273](/upload/website/prouct_img/normal/GTX637273/GTX637273_T-44760_20221014_WB_shRNA_watermark_22101717_269.webp "Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273")
![Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273](/upload/website/prouct_img/normal/GTX637273/GTX637273_T-44760_20220805_WB_22080923_205.webp "Anti-TFII-I antibody [HL1674] used in Western Blot (WB). GTX637273")
![Anti-TFII-I antibody [HL1674] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637273](/upload/website/prouct_img/normal/GTX637273/GTX637273_T-44760_20220916_ICC_IF_22102723_434.webp "Anti-TFII-I antibody [HL1674] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637273")