GeneTex

  • Country / Location Selection

United States (US)

TFR2 antibody [N3C2], Internal

Orthogonal
Anti-TFR2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110441
Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441
Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

Cat. No. GTX110441

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TFR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

K562 , HL-60

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TFR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transferrin receptor 2 , HFE3 , TFRC2

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Isoform Beta: Cytoplasm (Probable)

Background

This gene is a member of the transferrin receptor-like family and encodes a single-pass type II membrane protein with a protease associated (PA) domain, an M28 peptidase domain and a transferrin receptor-like dimerization domain. This protein mediates cellular uptake of transferrin-bound iron and mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TFR2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110441

GTX110441 IHC-P Image

TFR2 antibody [N3C2], Internal detects TFR2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TFR2 antibody [N3C2], Internal (GTX110441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

GTX110441 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFR2 antibody [N3C2], Internal (GTX110441) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 19254567

Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

GTX110441 WB Image

Sample (30 ug of whole cell lysate)
A: K562
B: HL-60
7.5% SDS PAGE
GTX110441 diluted at 1:1000

REFERENCE

There are currently no references for TFR2 antibody [N3C2], Internal (GTX110441). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for TFR2 antibody [N3C2], Internal (GTX110441). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169