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TGF beta 1 antibody [7F6]

Orthogonal
Anti-TGF beta 1 antibody [7F6] used in Flow cytometry (FACS). GTX60614
Anti-TGF beta 1 antibody [7F6] used in ELISA (ELISA). GTX60614
Anti-TGF beta 1 antibody [7F6] used in IHC (Paraffin sections) (IHC-P). GTX60614
Anti-TGF beta 1 antibody [7F6] used in Western Blot (WB). GTX60614
Anti-TGF beta 1 antibody [7F6] used in IHC (Paraffin sections) (IHC-P). GTX60614
Anti-TGF beta 1 antibody [7F6] used in Western Blot (WB). GTX60614

Cat. No. GTX60614

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F6

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all TGF beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human TGFb1 (AA: 62-195) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887989

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor beta 1 , CED , DPD1 , IBDIMDE , LAP , TGFB , TGFbeta

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.[provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-TGF beta 1 antibody [7F6] used in Flow cytometry (FACS). GTX60614

GTX60614 FACS Image

FACS analysis of A549 cells using GTX60614 TGF beta 1 antibody [7F6].
Green : TGF beta 1
Red : negative control

Anti-TGF beta 1 antibody [7F6] used in ELISA (ELISA). GTX60614

GTX60614 ELISA Image

ELISA analysis of antigen using GTX60614 TGF beta 1 antibody [7F6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-TGF beta 1 antibody [7F6] used in IHC (Paraffin sections) (IHC-P). GTX60614

GTX60614 IHC-P Image

IHC-P analysis of human lymphoid tissue using GTX60614 TGF beta 1 antibody [7F6].

Anti-TGF beta 1 antibody [7F6] used in Western Blot (WB). GTX60614

GTX60614 WB Image

WB analysis of human TGF beta 1 recombinant protein using GTX60614 TGF beta 1 antibody [7F6].

Anti-TGF beta 1 antibody [7F6] used in IHC (Paraffin sections) (IHC-P). GTX60614

GTX60614 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60614 TGF beta 1 antibody [7F6].

Anti-TGF beta 1 antibody [7F6] used in Western Blot (WB). GTX60614

GTX60614 WB Image

Orthogonal

The data was published in the journal Front Oncol in 2019.PMID: 31824844

REFERENCE

Application Reference

Ye J et al. Front Oncol 2019; 9 : 1251 miR-4666-3p and miR-329 Synergistically Suppress the Stemness of Colorectal Cancer Cells via Targeting TGF-帣/Smad Pathway.
Application : WB, FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409