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TGG1 / Myrosinase 1 antibody

Anti-TGG1 / Myrosinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00932
Anti-TGG1 / Myrosinase 1 antibody used in Western Blot (WB). GTX00932

Cat. No. GTX00932

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, EM, IHC

Reactivity

 Arabidopsis thaliana
Package
200 μg ($419)
See all TGG1 / Myrosinase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF Assay dependent
ELISA Assay dependent
EM 1:1000-1:2500
IHC 1:500-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Arabidopsis thaliana

Immunogen

A synthetic peptide, AQNNQTIVPSDVHT, corresponding to TGG1 protein (353-366) of A. thaliana, conjugated with bovine serum albumin

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Vacuole

Database

Research Area

DATA IMAGES

Anti-TGG1 / Myrosinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00932

GTX00932 ICC/IF Image

ICC/IF analysis of Arabidopsis rosette leaves (48-day-old plants) using GTX00932 TGG1 / Myrosinase 1 antibody. Asterisks show myrosin cells.
CW: cell wall; V: vacuole
(a, c): stained with CBB
(b,d): Primary antibody
c and d are enlarged images of the boxed area of a and b, respectivey.
(e): Ultrathin sections were mounted on Formvar-coated nicke grid by immunoelectron microscopy.
Dilution : 1:1000

Anti-TGG1 / Myrosinase 1 antibody used in Western Blot (WB). GTX00932

GTX00932 WB Image

WB analysis of Arabidopsis leaf extract using GTX00932 TGG1 / Myrosinase 1 antibody. Molecular mass of TGG1 is 61 kDa from the amino acid sequence. The protein undergoes modifications such as elimination of signal peptide and glycosylation at 9 positions, which chages molecular mass in mature form.
Dilution : 1:1000
Loading : 10 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 419