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TIM-1 antibody

Orthogonal
Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12016
Anti-TIM-1 antibody used in Western Blot (WB). GTX12016
Anti-TIM-1 antibody used in Western Blot (WB). GTX12016

Cat. No. GTX12016

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Rat
Package
100 μg ($429)
See all TIM-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of rat TIM 1(289-307aa HPRAEDNIYIIEDRSRGAE).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885468

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatitis A virus cellular receptor 1 , KIM-1 , Kim1

Cellular Localization

Membrane

Background

may be a member of the mucosal addressin cell adhesion molecule family; may be involved in restoration of the morphological integrity and function to postischemic kidney [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX12016

GTX12016 IHC-P Image

IHC-P analysis of rat testis tissue using GTX12016 TIM-1 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX12016

GTX12016 WB Image

WB analysis of various samples using GTX12016 TIM-1 antibody.
Lane 1 : rat kidney tissue lysates
Lane 2 : rat testis tissue lysates
Lane 3 : rat heart tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-TIM-1 antibody used in Western Blot (WB). GTX12016

GTX12016 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Int J Mol Sci 2019; 20 (22) A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 429