APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89675-PEP).
Product Note
This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CDVRTVLEHYALEDD, from the internal region of the protein sequence according to NP_001001563.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
translocase of inner mitochondrial membrane 50 , MGCA9 , TIM50 , TIM50L
Cellular Localization
Mitochondrion inner membrane
Background
This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
|
GTX89675 WB Image
WB analysis of human heart lysate using GTX89675 TIMM50 antibody, Internal. Dilution : 0.5μg/ml Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for TIMM50 antibody, Internal (GTX89675). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TIMM50 antibody, Internal (GTX89675). Be the first to share your experience with this product.