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TKTL1 antibody [N1C1]

Orthogonal
Anti-TKTL1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109459
Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459
Anti-TKTL1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109459
Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459
Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459

Cat. No. GTX109459

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TKTL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TKTL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038129

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transketolase like 1 , TKR , TKT2

Database

Research Area

DATA IMAGES

Anti-TKTL1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109459

GTX109459 ICC/IF Image

TKTL1 antibody [N1C1] detects TKTL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TKTL1 protein stained by TKTL1 antibody [N1C1] (GTX109459) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459

GTX109459 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TKTL1 antibody [N1C1] (GTX109459) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TKTL1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109459

GTX109459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Transketolase-like protein 1(GTX109459) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459

GTX109459 WB Image

Orthogonal

The data was published in the journal Aging Cell in 2014. PMID: 24757718

Anti-TKTL1 antibody [N1C1] used in Western Blot (WB). GTX109459

GTX109459 WB Image

The data was published in the journal J Exp Clin Cancer Res in 2019. PMID: 30971297

REFERENCE

Application Reference

Qin Z et al. J Exp Clin Cancer Res 2019; 38 (1) : 154 Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.
Application : WB
Reactivity : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
Application : IHC-P
Reactivity : Human
Li B et al. Aging Cell 2014; 13 (2) : 367-78 Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells.
Yuan SS et al. J Natl Cancer Inst 2014; 13 (2) : 367-378 Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169