The antibody recognizes the murine TLR4/MD-2 complex and is not useful for detection of TLR4 alone. The antibody can be used for flow cytometry and immunoprecipitation. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production.FLOW: 2-5 ug/ 1x 10^6 cellsIP: 2-5 ug/ ml: Please see Akashi et al (2000) for detailsFunctional Studies: Please see Akashi et al (2000) for details
Only detects murine TLR-4 when complexed to MD-2.
PBS with 0.1% BSA 0.02% sodium azide pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5mg/ml(Please refer to the vial label for the specific concentration.)
Mouse Ba/F3 cells expressing mouse TLR4/MD-2
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Toll-Like Receptor 4,Lps,Ly87,Ran/M1,Rasl2-8,Tlr4
Type I membrane protein.
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The MTS510 monoclonal antibody reacts preferentially, especially in flow cytometry, with murine TLR4 that is associated with MD-2. MTS510 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to co-precipitate MD-2 (30 kDa) with TLR4 (100 kDa).