GeneTex
United States (US)

TLR4 antibody [MTS510] (Biotin)

Cat No. GTX29105

Host Rat
Clonality Monoclonal
Clone Name MTS510
Isotype IgG2a
Application FACS, IP
Reactivity Mouse
APPLICATION

Application Note

The antibody recognizes the murine TLR4/MD-2 complex and is not useful for detection of TLR4 alone. The antibody can be used for flow cytometry and immunoprecipitation. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production.FLOW: 2-5 ug/ 1x 10^6 cellsIP: 2-5 ug/ ml: Please see Akashi et al (2000) for detailsFunctional Studies: Please see Akashi et al (2000) for details

Calculated MW

96 kDa. ( Note )

Specificity/Sensitivity

Only detects murine TLR-4 when complexed to MD-2.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.1% BSA 0.02% sodium azide pH7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse Ba/F3 cells expressing mouse TLR4/MD-2

Purification

IgG fraction

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Toll-Like Receptor 4,Lps,Ly87,Ran/M1,Rasl2-8,Tlr4

Cellular Localization

Type I membrane protein.

Background

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The MTS510 monoclonal antibody reacts preferentially, especially in flow cytometry, with murine TLR4 that is associated with MD-2. MTS510 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to co-precipitate MD-2 (30 kDa) with TLR4 (100 kDa).

Database

Research Area