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TLR9 antibody [N2], N-term

Overexpression
Anti-TLR9 antibody [N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100726
Anti-TLR9 antibody [N2], N-term used in Western Blot (WB). GTX100726
Anti-TLR9 antibody [N2], N-term used in Western Blot (WB). GTX100726
Anti-TLR9 antibody [N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100726

Cat. No. GTX100726

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TLR9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

THP-1 , TLR9 transfected 293T

Predict Reactivity

Sheep, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human TLR9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 9 , CD289

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TLR9 antibody [N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100726

GTX100726 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using TLR9(GTX100726) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TLR9 antibody [N2], N-term used in Western Blot (WB). GTX100726

GTX100726 WB Image

Sample (30 ug of whole cell lysate)

A: THP-1

7.5% SDS PAGE

GTX100726 diluted at 1:1000

Anti-TLR9 antibody [N2], N-term used in Western Blot (WB). GTX100726

GTX100726 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: TLR9 transfected 293T lysates
7.5% SDS PAGE
GTX100726 diluted at 1:2000

Anti-TLR9 antibody [N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100726

GTX100726 IHC-P Image

TLR9 antibody [N2], N-term detects TLR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TLR9 stained by TLR9 antibody [N2], N-term (GTX100726) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

E Fletcher et al. Transl Res 2023; S1931-5244 (23) : 00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169