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TMEM166 antibody

Anti-TMEM166 antibody used in Flow cytometry (FACS). GTX04091
Anti-TMEM166 antibody used in Western Blot (WB). GTX04091
Anti-TMEM166 antibody used in IHC (Paraffin sections) (IHC-P). GTX04091
Anti-TMEM166 antibody used in IHC (Paraffin sections) (IHC-P). GTX04091

Cat. No. GTX04091

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all TMEM166 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human TMEM166/EVA1A (MRLPLSHSPEHVEMALLSNILAAYSFVSENPERA).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eva-1 homolog A, regulator of programmed cell death , FAM176A , TMEM166

Cellular Localization

Endoplasmic reticulum membrane , Lysosome membrane

Database

Research Area

DATA IMAGES

Anti-TMEM166 antibody used in Flow cytometry (FACS). GTX04091

GTX04091 FACS Image

FACS analysis of A549 cells using GTX04091 TMEM166 antibody.
Dilution : 1μg/1x106
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-TMEM166 antibody used in Western Blot (WB). GTX04091

GTX04091 WB Image

WB analysis of various cell lysates using GTX04091 TMEM166 antibody.
Lane 1 : HepG2 cells
Lane 2 : PC-3 cells
Loading : 50 μg
Dilution : 0.5 μg/mL

Anti-TMEM166 antibody used in IHC (Paraffin sections) (IHC-P). GTX04091

GTX04091 IHC-P Image

IHC-P analysis of human renal cancer tissue using GTX04091 TMEM166 antibody.
Antigen retrieval : Heat mediated antigen retrieval in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1 μg/mL

Anti-TMEM166 antibody used in IHC (Paraffin sections) (IHC-P). GTX04091

GTX04091 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04091 TMEM166 antibody.
Antigen retrieval : Heat mediated antigen retrieval in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 429