APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
FACS
Cells were fixed in PBA containing 0.2 % formaldehyde before staining.
Neutralizing/Inhibition
Antibody V1q functions as a neutralizing antibody. The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody stains the extracellular domain of mouse TNF-α. The monoclonal antibody V1q recognizes both natural and recombinant TNF-α and shows neutralizing activity.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Cytotoxin purified from conA induced T cell clone 29
Purification
Purified IgD
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f
Cellular Localization
Cell membrane; Single-pass type II membrane protein
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Database
Research Area
REFERENCE
There are currently no references for TNF alpha antibody [V1q] (GTX54382). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TNF alpha antibody [V1q] (GTX54382). Be the first to share your experience with this product.
