GeneTex

  • Country / Location Selection

United States (US)

TNF alpha antibody

Anti-TNF alpha antibody used in Western Blot (WB). GTX48753
Anti-TNF alpha antibody used in Western Blot (WB). GTX48753

Cat. No. GTX48753

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Pig
Package
50 μg ($399)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA 1:2000-1:2500
IHC Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNF alpha.

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , TNFa

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody used in Western Blot (WB). GTX48753

GTX48753 WB Image

Western blot using GeneTex's protein-A purified anti-pig TNFa antibody shows detection of recombinant pig TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 1 μg/mL primary antibody overnight at 4ºC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.

Anti-TNF alpha antibody used in Western Blot (WB). GTX48753

GTX48753 WB Image

WB analysis of recombinant pig TNF alpha protein using GTX48753 TNF alpha antibody.
Dilution : 1 μg/mL

REFERENCE

There are currently no references for TNF alpha antibody (GTX48753). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for TNF alpha antibody (GTX48753). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399