APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2 μg/mL |
2.5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Brain Tissue Lysate(GTX27934)
Product Note
At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TOM70 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOM70.The immunogen is located within amino acids 50 - 100 of TOM70.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
translocase of outer mitochondrial membrane 70 , TOMM70A , Tom70
Cellular Localization
Mitochondrion outer membrane
Background
The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85358 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85358 TOM70 antibody. Working concentration : 5 μg/ml
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GTX85358 WB Image
WB analysis of mouse brain tissue lysate using GTX85358 TOM70 antibody. Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX85358 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85358 TOM70 antibody. Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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