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TRADD antibody [N2C3]

Comparison
Anti-TRADD antibody [N2C3] used in Western Blot (WB). GTX113042
Anti-TRADD antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113042

Cat. No. GTX113042

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRADD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRADD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952367

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNFRSF1A associated via death domain , Hs.89862

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRADD antibody [N2C3] used in Western Blot (WB). GTX113042

GTX113042 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TRADD antibody [N2C3] (GTX113042) diluted at 1:500 and competitor's antibody (sc-7868) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TRADD antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX113042

GTX113042 IHC-P Image

TRADD antibody [N2C3] detects TRADD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
TRADD stained by TRADD antibody [N2C3] (GTX113042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169