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TRAP1 antibody

KO/KD Orthogonal
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102017
Anti-TRAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017
Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

Cat. No. GTX102017

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241409

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor associated protein 1 , HSP 75 , HSP75 , HSP90L , TRAP-1

Cellular Localization

Mitochondrion

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

KO/KD

Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102017

GTX102017 IHC-P Image

TRAP1 antibody detects TRAP1 protein at mitochondrion on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TRAP1 antibody (GTX102017) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102017

GTX102017 ICC/IF Image

TRAP1 antibody detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TRAP1 protein stained by TRAP1 antibody (GTX102017) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
7.5% SDS PAGE
GTX102017 diluted at 1:1500

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 28397817

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017.PMID: 28397817

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Orthogonal

The data was published in the 2022 in J Biomed Sci. PMID: 35765067

Anti-TRAP1 antibody used in Western Blot (WB). GTX102017

GTX102017 WB Image

Orthogonal

The data was published in the 2022 in J Biomed Sci. PMID: 35765067

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : WB, PLA
Reactivity : Human
Matassa DS et al. Cancer Cell Int 2022; 22 (1) : 402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
Application : PLA
Reactivity : Human
Jia-Huang Chen et al. J Biomed Sci 2022; 29 (1) : 46 The down-regulation of XBP1, an unfolded protein response effector, promotes acute kidney injury to chronic kidney disease transition.
Yool Lee et al. Sci Adv 2021; 7 (7) Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Avolio R et al. Genome Res 2023; 33 (8):1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : WB, PLA
Reactivity : Human
Matassa DS et al. Cancer Cell Int 2022; 22 (1):402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
Application : PLA
Reactivity : Human
Jia-Huang Chen et al. J Biomed Sci 2022; 29 (1):46 The down-regulation of XBP1, an unfolded protein response effector, promotes acute kidney injury to chronic kidney disease transition.
Yool Lee et al. Sci Adv 2021; 7 (7) Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.
Application : WB
Reactivity : Human
Lanzillotta C et al. Prog Neurobiol 2020;:101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Zhao X et al. Sci Rep 2017; 7:46344 Tenofovir and * down-regulate mitochondrial chaperone TRAP1 and succinate dehydrogenase subunit B to metabolically reprogram glucose metabolism and induce nephrotoxicity.
Application : WB
Reactivity : Mouse
Brauers E et al. Proteomics Clin Appl 2016; (Epub) The Caveolin-3 G56S sequence variant of unknown significance: Muscle biopsy findings and functional cell biological analysis.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production