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TRH Receptor antibody [C2C3], C-term

Anti-TRH Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100030
Anti-TRH Receptor antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100030
Anti-TRH Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100030
Anti-TRH Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100030

Cat. No. GTX100030

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human TRH Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241388

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thyrotropin releasing hormone receptor , TRH-R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Thyrotropin-releasing hormone (TRH; MIM 275120), a small neuropeptide, is widely distributed throughout the central and peripheral nervous system as well as in extraneural tissues. The peptide is synthesized in the hypothalamus and transported by the portal vascular system to the anterior pituitary where it acts on thyrotropic and lactotropic cells to promote secretion of TSH and prolactin, respectively. Thyrotropin-releasing hormone receptor is a G protein-coupled receptor that activates the inositol phospholipid-calcium-protein kinase C transduction pathway upon the binding of TRH. The TRHR gene is expressed in the thyrotrope cells of the anterior pituitary.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TRH Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100030

GTX100030 IHC-P Image

TRH Receptor antibody [C2C3], C-term detects TRH Receptor protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TRH Receptor stained by TRH Receptor antibody [C2C3], C-term (GTX100030) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRH Receptor antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100030

GTX100030 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using TRHR(GTX100030) antibody at 1:500 dilution.

Anti-TRH Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100030

GTX100030 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100030 diluted at 1:2000

Anti-TRH Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100030

GTX100030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using TRHR(GTX100030) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for TRH Receptor antibody [C2C3], C-term (GTX100030). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TRH Receptor antibody [C2C3], C-term (GTX100030). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169