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TRIM71 antibody

Anti-TRIM71 antibody used in IHC (Paraffin sections) (IHC-P). GTX85001
Anti-TRIM71 antibody used in IHC (Paraffin sections) (IHC-P). GTX85001
Anti-TRIM71 antibody used in Western Blot (WB). GTX85001

Cat. No. GTX85001

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TRIM71 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71.The immunogen is located within amino acids 680 - 730 of TRIM71.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 71 , LIN-41 , LIN41

Background

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development , and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

Database

Research Area

DATA IMAGES

Anti-TRIM71 antibody used in IHC (Paraffin sections) (IHC-P). GTX85001

GTX85001 IHC-P Image

IHC-P analysis of human brain tissue using GTX85001 TRIM71 antibody.
Working concentration : 20 μg/ml

Anti-TRIM71 antibody used in IHC (Paraffin sections) (IHC-P). GTX85001

GTX85001 IHC-P Image

IHC-P analysis of human brain tissue using GTX85001 TRIM71 antibody.
Working concentration : 5 μg/ml

Anti-TRIM71 antibody used in Western Blot (WB). GTX85001

GTX85001 WB Image

WB analysis of human brain tissue lysate using GTX85001 TRIM71 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399