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TUFM antibody [N3C3]

Anti-TUFM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101764
Anti-TUFM antibody [N3C3] used in Immunoprecipitation (IP). GTX101764
Anti-TUFM antibody [N3C3] used in Western Blot (WB). GTX101764

Cat. No. GTX101764

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, PLA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TUFM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TUFM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241421

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Tu translation elongation factor, mitochondrial , COXPD4 , EF-TuMT , EFTU , P43

Background

This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TUFM antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101764

GTX101764 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU16 xenograft, using TUFM(GTX101764) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TUFM antibody [N3C3] used in Immunoprecipitation (IP). GTX101764

GTX101764 IP Image

TUFM antibody [N3C3] immunoprecipitates TUFM protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TUFM protein by 4 μg TUFM antibody [N3C3] (GTX101764)
10 % SDS-PAGE
The immunoprecipitated TUFM protein was detected by TUFM antibody [N3C3] (GTX101764) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-TUFM antibody [N3C3] used in Western Blot (WB). GTX101764

GTX101764 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101764 diluted at 1:2000

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : PLA
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169