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TXNL2 antibody [N2C3]

KO/KD
Anti-TXNL2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118339
Anti-TXNL2 antibody [N2C3] used in Western Blot (WB). GTX118339
Anti-TXNL2 antibody [N2C3] used in Western Blot (WB). GTX118339
Anti-TXNL2 antibody [N2C3] used in Immunoprecipitation (IP). GTX118339
Anti-TXNL2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118339

Cat. No. GTX118339

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TXNL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Mouse brain , Raji

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TXNL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617991

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaredoxin 3 , GLRX4 , GRX3 , GRX4 , PICOT , TXNL2 , TXNL3

Cellular Localization

Cytoplasm , cell cortex

Background

This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TXNL2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX118339

GTX118339 IHC-P Image

TXNL2 antibody [N2C3] detects TXNL2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TXNL2 antibody [N2C3] (GTX118339) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TXNL2 antibody [N2C3] used in Western Blot (WB). GTX118339

GTX118339 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX118339 diluted at 1:10000

Anti-TXNL2 antibody [N2C3] used in Western Blot (WB). GTX118339

GTX118339 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX118339 diluted at 1:1000

Anti-TXNL2 antibody [N2C3] used in Immunoprecipitation (IP). GTX118339

GTX118339 IP Image

Immunoprecipitation of TXNL2 protein from 293T whole cell extracts using 5 μg of TXNL2 antibody [N2C3] (GTX118339).
Western blot analysis was performed using TXNL2 antibody [N2C3] (GTX118339).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TXNL2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118339

GTX118339 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using TXNL2(GTX118339) antibody at 1:200 dilution.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169