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Talin-1 antibody, Internal

Anti-Talin-1 antibody, Internal used in Western Blot (WB). GTX88357

Cat. No. GTX88357

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μg ($399)
See all Talin-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88357-PEP).

Product Note

This antibody is not expected to cross-react with the highly similar protein Talin 2.

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QEALHTQMLTAVQE, from the internal region of the protein sequence according to NP_006280.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10732152

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

talin 1 , ILWEQ , TLN , talin-1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-Talin-1 antibody, Internal used in Western Blot (WB). GTX88357

GTX88357 WB Image

WB analysis of human peripheral mononucleocytes lysate using GTX88357 Talin-1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Beaty BT et al. J Cell Biol 2014; (Epub) Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis.
Application : WB, ICC/IF, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399