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Thrombin Receptor antibody [N1N2], N-term

Orthogonal
Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035
Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035
Anti-Thrombin Receptor antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116035
Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035

Cat. No. GTX116035

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Thrombin Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Thrombin Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor II thrombin receptor , CF2R , HTR , PAR-1 , PAR1 , TR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035

GTX116035 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thrombin Receptor antibody [N1N2], N-term (GTX116035) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035

GTX116035 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Thrombin Receptor antibody [N1N2], N-term (GTX116035) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin Receptor antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116035

GTX116035 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Thrombin Receptor(GTX116035) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Thrombin Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX116035

GTX116035 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Thrombin Receptor antibody [N1N2], N-term (GTX116035) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169