Thrombospondin 2 antibody [HL1140]

Cat. No. GTX636411

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1140
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Thrombospondin 2 products

Datasheet

View product citations for antibody GTX636411 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX636506, Detection : GTX636411 or Capture : GTX636411, Detection : GTX636506. Please notice that the detected antibody needs to be conjugated to HRP when paired with the capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombospondin 2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 2 , TSP2

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7058 THBS2
UniProt: P35442 THBS2

Research Area

DATA IMAGES

GTX636411 WB Image

AsPC-1 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636411 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1140] (GTX636411) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (GTX636506) (1 μg/mL)
Please notice that GTX636506 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636411. Please contact us for custom HRP-conjugated antibody.

GTX636411 IHC-P Image

Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636411 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636411 ELISA Image

Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1194] (GTX636506) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (GTX636411) (1 μg/mL)
Please notice that GTX636411 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636506. Please contact us for custom HRP-conjugated antibody.

GTX636411 ICC/IF Image

Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Thrombospondin 2 antibody [HL1140]

Cat. No. GTX636411

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX636411 WB Image

GTX636411 ELISA Image

GTX636411 IHC-P Image

GTX636411 WB Image

GTX636411 ELISA Image

GTX636411 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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