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Transcription Termination Factor 1 antibody

Anti-Transcription Termination Factor 1 antibody used in Western Blot (WB). GTX129804
Anti-Transcription Termination Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129804
Anti-Transcription Termination Factor 1 antibody used in Immunoprecipitation (IP). GTX129804

Cat No. GTX129804

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

293T , 293T NE
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transcription Termination Factor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

transcription termination factor 1 , TTF-1 , TTF-I

Cellular Localization

Nucleus , nucleolus

Background

This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011]

Database

DATA IMAGES
Anti-Transcription Termination Factor 1 antibody used in Western Blot (WB). GTX129804

GTX129804 WB Image

TTF1 antibody detects TTF1 protein by western blot analysis. 293T whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TTF1 antibody (GTX129804) diluted by 1:1000 and developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Transcription Termination Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129804

GTX129804 IHC-P Image

Transcription Termination Factor 1 antibody detects Transcription Termination Factor 1 protein at nucleus in human small cell lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human small cell lung cancer.
Transcription Termination Factor 1 antibody (GTX129804) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transcription Termination Factor 1 antibody used in Immunoprecipitation (IP). GTX129804

GTX129804 IP Image

Immunoprecipitation of Transcription Termination Factor 1 protein from 293T whole cell extracts using 5 μg of Transcription Termination Factor 1 antibody (GTX129804).
Western blot analysis was performed using Transcription Termination Factor 1 antibody (GTX129804).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169