GeneTex
United States (US)

Transferrin antibody (HRP)

Cat No. GTX29036

Host Sheep
Clonality Polyclonal
Isotype IgG
Application EIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
EIA 1:3,200-1:6,400

Note :

EIA
2 μg/ml transferrin and TMB as a substrate

Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Specificity/Sensitivity

The unconjugated immunoglobulin gives a single arc when tested by IEP against human serum. Identity hasbeen confirmed by double diffusion (Ouchterlony) against human serum and an anti-human transferrin ofknown specificity. Transferrin is 79 kDa a single polypeptide chain glycoprotein, and is a member of theiron binding family of proteins.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 0.05% ProclinTM300, 0.02% Methylisothiazolone, 0.02% Bromonitrodioxane

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human holo-transferrin purified from human serum, shown to be homogeneous by SDS-PAGE.

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transferrin,Hel-S-71P,Pro1557,Pro2086,Tfqtl1,Tf

Cellular Localization

Secreted

Background

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Database

Research Area