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Transglutaminase 2 antibody [N1N3]

Anti-Transglutaminase 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111701
Anti-Transglutaminase 2 antibody [N1N3] used in Western Blot (WB). GTX111701
Anti-Transglutaminase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111701
Anti-Transglutaminase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111701
Anti-Transglutaminase 2 antibody [N1N3] used in Western Blot (WB). GTX111701

Cat. No. GTX111701

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Transglutaminase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rat, Bovine, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transglutaminase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618362

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transglutaminase 2 , TG(C) , TGC

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transglutaminase 2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111701

GTX111701 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Transglutaminase 2(GTX111701) antibody at 1:500 dilution.

Anti-Transglutaminase 2 antibody [N1N3] used in Western Blot (WB). GTX111701

GTX111701 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX111701 diluted at 1:2000

Anti-Transglutaminase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111701

GTX111701 IHC-P Image

Transglutaminase 2 antibody [N1N3] detects Transglutaminase 2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Transglutaminase 2 antibody [N1N3] (GTX111701) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transglutaminase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111701

GTX111701 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Transglutaminase 2(GTX111701) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transglutaminase 2 antibody [N1N3] used in Western Blot (WB). GTX111701

GTX111701 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N1N3] (GTX111701) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169