APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1μg/ml |
| 2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX13574-PEP).
Product Note
This antibody is expected to recognise reported isoform 1 (NP_003321.2), isoform 2 (NP_877393.1; NP_877419.1; NP_877420.1) and isoform 3 (NP_001087240.1)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence TKRSGASILQAGC, from the C Terminus of the protein sequence according to NP_003321.2; NP_877393.1; NP_877419.1; NP_877420.1; NP_001087240.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017]
Database
Research Area
DATA IMAGES
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GTX13574 WB Image
WB analysis of human placenta lysate using GTX13574 TrxR1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX13574 IHC-P Image
IHC-P analysis of human spleen using GTX13574 TrxR1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX13574 IHC-P Image
IHC-P analysis of human testis using GTX13574 TrxR1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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REFERENCE
There are currently no references for TrxR1 antibody, C-term (GTX13574). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TrxR1 antibody, C-term (GTX13574). Be the first to share your experience with this product.
