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U2AF65 antibody

Anti-U2AF65 antibody used in Immunoprecipitation (IP). GTX55828
Anti-U2AF65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55828
Anti-U2AF65 antibody used in Western Blot (WB). GTX55828

Cat. No. GTX55828

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all U2AF65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2 (NP_001012496.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 2 , U2AF65

Cellular Localization

Nucleus

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-U2AF65 antibody used in Immunoprecipitation (IP). GTX55828

GTX55828 IP Image

IP analysis of SW620 cell lysate using GTX55828 U2AF65 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:500

Anti-U2AF65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55828

GTX55828 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55828 U2AF65 antibody.
Blue : DAPI

Anti-U2AF65 antibody used in Western Blot (WB). GTX55828

GTX55828 WB Image

WB analysis of various sample lysates using GTX55828 U2AF65 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399