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UBA2 antibody [N1N3]

Anti-UBA2 antibody [N1N3] used in Western Blot (WB). GTX102613
Anti-UBA2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102613

Cat. No. GTX102613

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UBA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UBA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617314

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like modifier activating enzyme 2 , ARX , HRIHFB2115 , SAE2

Cellular Localization

Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-UBA2 antibody [N1N3] used in Western Blot (WB). GTX102613

GTX102613 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
UBA2 antibody
GTX102613 diluted at 1:1000

Anti-UBA2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102613

GTX102613 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBA2(GTX102613) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169