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UBA6 antibody

Anti-UBA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX65920
Anti-UBA6 antibody used in Immunoprecipitation (IP). GTX65920
Anti-UBA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65920
Anti-UBA6 antibody used in Western Blot (WB). GTX65920

Cat. No. GTX65920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all UBA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like modifier activating enzyme 6 , E1-L2 , MOP-4 , UBE1L2

Background

Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 [PubMed 17597759]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-UBA6 antibody used in IHC (Paraffin sections) (IHC-P). GTX65920

GTX65920 IHC-P Image

IHC-P analysis of human kidney tissue using GTX65920 UBA6 antibody.
Dilution : 1:100

Anti-UBA6 antibody used in Immunoprecipitation (IP). GTX65920

GTX65920 IP Image

IP analysis of 293T cell lysate using GTX65920 UBA6 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-UBA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65920

GTX65920 ICC/IF Image

ICC/IF analysis of A549 cells using GTX65920 UBA6 antibody.
Blue : DAPI

Anti-UBA6 antibody used in Western Blot (WB). GTX65920

GTX65920 WB Image

WB analysis of various sample lysates using GTX65920 UBA6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399