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UBE2B antibody

Anti-UBE2B antibody used in Western Blot (WB). GTX100416
Anti-UBE2B antibody used in Western Blot (WB). GTX100416
Anti-UBE2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100416

Cat. No. GTX100416

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBE2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse brain , 3xFlag-human UBE2B-transfected 293T

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241425

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 B , E2-17kDa , HHR6B , HR6B , RAD6B , UBC2

Cellular Localization

Cell membrane , Nucleus

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2B antibody used in Western Blot (WB). GTX100416

GTX100416 WB Image

UBE2B antibody detects UBE2B protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells
12 % SDS-PAGE
UBE2B antibody (GTX100416) dilution: 1:5000

Anti-UBE2B antibody used in Western Blot (WB). GTX100416

GTX100416 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX100416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UBE2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100416

GTX100416 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(GTX100416) antibody at 1:500 dilution.

REFERENCE

Application Reference

Wan-Chen Kao et al. Oncol Lett 2022; 23 (6) : 194 Role of high ubiquitin-conjugating enzyme E2 expression as a prognostic factor in nasopharyngeal carcinoma.
Hsu SH et al. Biochem Pharmacol 2018; 158 : 327-338 Ubiquitin-conjugating enzyme E2 B regulates the ubiquitination of O6-methylguanine-DNA methyltransferase and BCNU sensitivity in human nasopharyngeal carcinoma cells.
Cheng CM et al. J Pathol 2016; (Epub) Up-regulation of miR-455-5p by the TGF-β-SMAD signalling axis promotes the proliferation of oral squamous cancer cells by targeting UBE2B.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169