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UBE2C antibody [N1C3]

Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599
Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599
Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599
Anti-UBE2C antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100599

Cat. No. GTX100599

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBE2C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

UBE2C transfected 293T , NIH-3T3

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952480

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 C , UBCH10 , dJ447F3.2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599

GTX100599 WB Image

Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C polyclonal antibody.
A: Non-transfected lysate.
B: UBE2C transfected lysate.
12% SDS PAGE
GTX100599 diluted at 1:500

Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599

GTX100599 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with UBE2C antibody [N1C3] (GTX100599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UBE2C antibody [N1C3] used in Western Blot (WB). GTX100599

GTX100599 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
12% SDS PAGE

GTX100599 diluted at 1:1000

Anti-UBE2C antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100599

GTX100599 ICC/IF Image

UBE2C antibody [N1C3] detects UBE2C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE2C protein stained by UBE2C antibody [N1C3] (GTX100599) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169