APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human UBE2D1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952481
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 D1 , E2(17)KB1 , SFT , UBC4/5 , UBCH5 , UBCH5A
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109257 IHC-P Image
UBE2D1 antibody detects UBE2D1 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
UBE2D1 antibody (GTX109257) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109257 IHC-P Image
UBE2D1 antibody detects UBE2D1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
UBE2D1 antibody (GTX109257) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109257 IHC-P Image
Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using UBE2D1(GTX109257) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109257 WB Image
Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX109257 diluted at 1:1000
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GTX109257 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2D1(GTX109257) antibody at 1:200 dilution.
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REFERENCE
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REVIEW
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