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UBE2O antibody [C1C2], Internal

Anti-UBE2O antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119315
Anti-UBE2O antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119315
Anti-UBE2O antibody [C1C2], Internal used in Western Blot (WB). GTX119315
Anti-UBE2O antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119315

Cat. No. GTX119315

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBE2O products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

293T , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2O. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 O , E2-230K

Background

Catalyzes the covalent attachment of ubiquitin to other proteins.

Database

Research Area

DATA IMAGES

Anti-UBE2O antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119315

GTX119315 IHC-P Image

UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
UBE2O antibody [C1C2], Internal (GTX119315) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UBE2O antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119315

GTX119315 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using UBE2O(GTX119315) antibody at 1:500 dilution.

Anti-UBE2O antibody [C1C2], Internal used in Western Blot (WB). GTX119315

GTX119315 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with UBE2O antibody [C1C2], Internal (GTX119315) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UBE2O antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX119315

GTX119315 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using UBE2O(GTX119315) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Huang Y et al. Cell Death Differ 2020; (Epub) UBE2O targets Mxi1 for ubiquitination and degradation to promote lung cancer progression and radioresistance.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169