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UBE2S antibody [AT2C12]

Anti-UBE2S antibody [AT2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53797
Anti-UBE2S antibody [AT2C12] used in Western Blot (WB). GTX53797

Cat. No. GTX53797

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2C12

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all UBE2S products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human UBE2S (1-222aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 S , E2-EPF , E2EPF , EPF5

Background

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-UBE2S antibody [AT2C12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53797

GTX53797 ICC/IF Image

ICC/IF analysis of HeLa cells using UBE2S antibody at a dilution of 1:500 (green) and Phalloidin (red).

Anti-UBE2S antibody [AT2C12] used in Western Blot (WB). GTX53797

GTX53797 WB Image

WB analysis of 293T lysate (50ug) using UBE2S antibody at a dilution of 1:250.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399