APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE3A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885224
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin protein ligase E3A , ANCR , AS , E6-AP , EPVE6AP , HPVE6A
Cellular Localization
Nucleus
Background
This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
UBE3A antibody (GTX101092) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101092 ICC/IF Image
UBE3A antibody detects UBE3A protein at nucleus by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
UBE3A antibody (GTX101092) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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