APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
5-10μg/ml |
Note :
IHC-P
Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88365-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin protein ligase E3 component n-recognin 4 , RBAF600 , ZUBR1 , p600
Cellular Localization
Membrane , Cytoplasm , Cytoplasm, cytoskeleton , Nucleus
Background
The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]
Database
Research Area
DATA IMAGES
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GTX88365 IHC-P Image
IHC-P analysis of human spleen using GTX88365 UBR4 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
There are currently no references for UBR4 antibody, C-term (GTX88365). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UBR4 antibody, C-term (GTX88365). Be the first to share your experience with this product.