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UCP1 antibody

Orthogonal
Anti-UCP1 antibody used in Western Blot (WB). GTX112784
Anti-UCP1 antibody used in Western Blot (WB). GTX112784
Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784
Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784
Anti-UCP1 antibody used in Western Blot (WB). GTX112784
Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784
Anti-UCP1 antibody used in Western Blot (WB). GTX112784

Cat. No. GTX112784

Reference 13
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UCP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623171

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uncoupling protein 1 , SLC25A7 , UCP

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UCP1 antibody used in Western Blot (WB). GTX112784

GTX112784 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UCP1 antibody used in Western Blot (WB). GTX112784

GTX112784 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

GTX112784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UCP1(GTX112784) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

GTX112784 IHC-P Image

UCP1 antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
UCP1 stained by UCP1 antibody (GTX112784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UCP1 antibody used in Western Blot (WB). GTX112784

GTX112784 WB Image

Orthogonal

The data was published in the journal Nutrients in 2019. PMID: 30959933

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

GTX112784 IHC-P Image

Orthogonal

The data was published in the journal Nutrients in 2019. PMID: 30959933

Anti-UCP1 antibody used in Western Blot (WB). GTX112784

GTX112784 WB Image

Orthogonal

The data was published in the journal PLoS Biol in 2019. PMID: 30653498

REFERENCE

Application Reference

B Stojni? et al. et al. Biofactors 2023; Glycosaminoglycan dermatan sulfate supplementation decreases diet?induced obesity and metabolic dysfunction in mice
Application : IHC-P
Liao FH et al. Mol Pharm 2022; 19 (11) : 4299-4310 Transdermal Delivery of Succinate Accelerates Energy Dissipation of Brown Adipocytes to Reduce Remote Fat Accumulation.
Application : IHC-P
Reactivity : Mouse
Shinichi Morita et al. Sci Rep 2022; 12 (1) : 10105 Daisaikoto improves fatty liver and obesity in melanocortin-4 receptor gene-deficient mice via the activation of brown adipose tissue.
Application : IHC-P
Reactivity : Mouse
Alessio Molfino et al. Cancers (Basel) 2022; 14 (8) Evaluation of Browning Markers in Subcutaneous Adipose Tissue of Newly Diagnosed Gastrointestinal Cancer Patients with and without Cachexia.
Application : WB
Bojan Stojni? et al. Nutrients 2021; 13 (10)
Application : IHC-P
Reactivity : Mouse
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Submit a Reference

REVIEW

UCP1 antibody Cat. No. GTX112784

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-UCP1 antibody used in Western Blot (WB). GTX112784
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : brown fat
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Brown fat lysate from mouse housed in room temperature, Lane 2: after 4 oC cold exposure
Submit a Review
Application Reference
B Stojni? et al. et al. Biofactors 2023; Glycosaminoglycan dermatan sulfate supplementation decreases diet?induced obesity and metabolic dysfunction in mice
Application : IHC-P
Liao FH et al. Mol Pharm 2022; 19 (11):4299-4310 Transdermal Delivery of Succinate Accelerates Energy Dissipation of Brown Adipocytes to Reduce Remote Fat Accumulation.
Application : IHC-P
Reactivity : Mouse
Shinichi Morita et al. Sci Rep 2022; 12 (1):10105 Daisaikoto improves fatty liver and obesity in melanocortin-4 receptor gene-deficient mice via the activation of brown adipose tissue.
Application : IHC-P
Reactivity : Mouse
Alessio Molfino et al. Cancers (Basel) 2022; 14 (8) Evaluation of Browning Markers in Subcutaneous Adipose Tissue of Newly Diagnosed Gastrointestinal Cancer Patients with and without Cachexia.
Application : WB
Bojan Stojni? et al. Nutrients 2021; 13 (10)
Application : IHC-P
Reactivity : Mouse
Lu KY et al. Biochim Biophys Acta Mol Cell Biol Lipids 2021; 1866 (12):159033 N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes.
Application : WB, IHC-P
Reactivity : Mouse
Lu KY et al. Biomed Pharmacother 2021; 139:111687 3-N-butylphthalide protects against high-fat-diet-induced obesity in C57BL/6 mice and increases metabolism in lipid-accumulating cells.
Application : IHC-P
Reactivity : Mouse
Pilling D et al. Am J Pathol 2021; 191 (1):131-143 High-Fat Diet-Induced Adipose Tissue and Liver Inflammation and Steatosis in Mice Are Reduced by Inhibiting Sialidases.
Application : IHC-P
Reactivity : Mouse
Pilling D et al. Am J Pathol 2019; Serum Amyloid P and a Dendritic Cell-Specific Intercellular Adhesion Molecule-3-Grabbing Nonintegrin Ligand Inhibit High-Fat Diet-Induced Adipose Tissue and Liver Inflammation and Steatosis in Mice.
Application : IHC-P
Reactivity : Mouse
Gille A et al. Nutrients 2019; 11 (4) A Lipophilic Fucoxanthin-Rich Phaeodactylum tricornutum Extract Ameliorates Effects of Diet-Induced Obesity in C57BL/6J Mice.
Application : IHC-P
Reactivity : Mouse
Gohlke S et al. Mol Metab 2019; 24:1月17日 Identification of functional lipid metabolism biomarkers of brown adipose tissue aging.
Application : WB
Reactivity : Mouse
Song W et al. PLoS Biol 2019; 17 (1):e2006571 Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue.
Application : WB
Reactivity : Mouse
Zhang X et al. Cell Death Differ 2017; 24 (2):225-237 Hedgehog signaling in bone regulates whole-body energy metabolism through a bone-adipose endocrine relay mediated by PTHrP and adiponectin.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169