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URP2 antibody

Anti-URP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116928
Anti-URP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116928
Anti-URP2 antibody used in Western Blot (WB). GTX116928
Anti-URP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116928

Cat. No. GTX116928

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all URP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human URP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177849

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fermitin family member 3 , KIND3 , MIG-2 , MIG2B , UNC112C , URP2 , URP2SF

Cellular Localization

Cytoplasm (Probable) , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Kindlins are a small family of proteins that mediate protein-protein interactions involved in integrin activation and thereby have a role in cell adhesion, migration, differentiation, and proliferation. The protein encoded by this gene has a key role in the regulation of hemostasis and thrombosis. This protein may also help maintain the membrane skeleton of erythrocytes. Mutations in this gene cause the autosomal recessive leukocyte adhesion deficiency syndrome-III (LAD-III). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-URP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116928

GTX116928 ICC/IF Image

URP2 antibody detects URP2 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: URP2 protein stained by URP2 antibody (GTX116928) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-URP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116928

GTX116928 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using URP2(GTX116928) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-URP2 antibody used in Western Blot (WB). GTX116928

GTX116928 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
7.5% SDS PAGE
GTX116928 diluted at 1:1000

Anti-URP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116928

GTX116928 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using URP2(GTX116928) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169