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USF2 antibody

Comparison
Anti-USF2 antibody used in Western Blot (WB). GTX129239
Anti-USF2 antibody used in Western Blot (WB). GTX129239
Anti-USF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129239

Cat. No. GTX129239

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all USF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NIH-3T3 , Raw264.7 , C2C12 , Jurkat , Raji , NCI-H929

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885937

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

upstream transcription factor 2, c-fos interacting , FIP , bHLHb12

Cellular Localization

Nucleus

Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-USF2 antibody used in Western Blot (WB). GTX129239

GTX129239 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with USF2 antibody (GTX129239) diluted at 1:500.

Anti-USF2 antibody used in Western Blot (WB). GTX129239

GTX129239 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with USF2 antibody (GTX129239) diluted at 1:500 and competitor's antibody (SC-862) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-USF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129239

GTX129239 ICC/IF Image

USF2 antibody detects USF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USF2 stained by USF2 antibody (GTX129239) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

REFERENCE

Application Reference

Lee YK et al. Cancers (Basel) 2020; 12 (9) Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169