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USP25 antibody

Anti-USP25 antibody used in Western Blot (WB). GTX33576
Anti-USP25 antibody used in Immunoprecipitation (IP). GTX33576
Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX33576
Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX33576

Cat. No. GTX33576

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all USP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human USP25 (NP_037528.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 25 , USP21

Cellular Localization

Cytoplasm,Nucleus

Background

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-USP25 antibody used in Western Blot (WB). GTX33576

GTX33576 WB Image

WB analysis of various sample lysates using GTX33576 USP25 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-USP25 antibody used in Immunoprecipitation (IP). GTX33576

GTX33576 IP Image

IP analysis of HeLa cell lysate using GTX33576 USP25 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX33576

GTX33576 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33576 USP25 antibody.
Dilution : 1:100

Anti-USP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX33576

GTX33576 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33576 USP25 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399