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USP9X antibody

Orthogonal
Anti-USP9X antibody used in Western Blot (WB). GTX135634
Anti-USP9X antibody used in IHC (Paraffin sections) (IHC-P). GTX135634
Anti-USP9X antibody used in IHC (Paraffin sections) (IHC-P). GTX135634
Anti-USP9X antibody used in Western Blot (WB). GTX135634
Anti-USP9X antibody used in Western Blot (WB). GTX135634

Cat. No. GTX135634

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all USP9X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

292 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human USP9X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909862

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 9 X-linked , DFFRX , FAF , FAM , MRX99 , MRXS99F

Cellular Localization

Cytoplasm

Background

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-USP9X antibody used in Western Blot (WB). GTX135634

GTX135634 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP9X antibody (GTX135634) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-USP9X antibody used in IHC (Paraffin sections) (IHC-P). GTX135634

GTX135634 IHC-P Image

USP9X antibody detects USP9X protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
USP9X stained by USP9X antibody (GTX135634) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP9X antibody used in IHC (Paraffin sections) (IHC-P). GTX135634

GTX135634 IHC-P Image

USP9X antibody detects USP9X protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
USP9X stained by USP9X antibody (GTX135634) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP9X antibody used in Western Blot (WB). GTX135634

GTX135634 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP9X antibody (GTX135634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP9X antibody used in Western Blot (WB). GTX135634

GTX135634 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP9X antibody (GTX135634) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169