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UVSSA antibody [N1N2], N-term

Anti-UVSSA antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106751
Anti-UVSSA antibody [N1N2], N-term used in Western Blot (WB). GTX106751

Cat. No. GTX106751

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UVSSA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UVSSA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950618

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UV stimulated scaffold protein A , KIAA1530 , UVSS3

Database

Research Area

DATA IMAGES

Anti-UVSSA antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106751

GTX106751 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UVSSA antibody [N1N2], N-term (GTX106751) at 1:200 dilution.

Anti-UVSSA antibody [N1N2], N-term used in Western Blot (WB). GTX106751

GTX106751 WB Image

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
7.5% SDS PAGE
GTX106751 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liebau RC et al. bioRxiv 2023; Transcription-Coupled Repair of DNA Interstrand Crosslinks by UVSSA.
Kordon MM et al. Proc Natl Acad Sci U S A 2022; 119 (11) : e2116254119 The UVSSA protein is part of a genome integrity homeostasis network with links to transcription-coupled DNA repair and ATM signaling.
Application : WB
Reactivity : Human
Mai Sato et al. J Cell Biol 2021; 220 (2) The UVSSA complex alleviates MYC-driven transcription stress.
Application : WB
Reactivity : Human
Higa M et al. FEBS J 2018; (Epub) Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7.
Application : WB
Reactivity : Human
Higa M et al. J Biol Chem 2016; (Epub) Stabilization of UV-stimulated Scaffold Protein A by Interaction with Ubiquitin-specific Peptidase 7 is Essential for Transcription-coupled Nucleotide Excision Repair.
Application : WB
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169