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Ulp1 antibody

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820
Anti-Ulp1 antibody used in Western Blot (WB). GTX48820
Anti-Ulp1 antibody used in Western Blot (WB). GTX48820
Anti-Ulp1 antibody used in Western Blot (WB). GTX48820
Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

Cat. No. GTX48820

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Yeast
Package
100 μg ($399)
See all Ulp1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
ELISA 1:4000-1:20000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Recombinant yeast ULP-1 protein.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUMO protease ULP1 , NIB1

Database

Research Area

DATA IMAGES

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

GTX48820 WB Image

Western Blot of Rabbit Anti-ULP1 Antibody. (in duplicate) Lane 1: 50ng ULP1CD. Lane 2: 25ng ULP1CD. Lane 3: 12.5ng ULP1CD. Lane 4: 6.25ng ULP1CD. Lane 5: 3.13ng ULP1CD. Primary Antibody: Anti-ULP1 at 1:1,000 dilution O/N at 4ºC. Secondary Antibody: Goat Anti-Rabbit HRP 1:5,000 RT for 30 min.

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

GTX48820 WB Image

Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TBST, and probed overnight at 4ºC with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TBST. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

GTX48820 WB Image

WB analysis of recombinant yeast Ulp1 protein using GTX48820 Ulp1 antibody.
Lane 1 : 50 ng
Lane 2 : 25 ng
Lane 3 : 12.5 ng
Lane 4 : 6.25 ng
Lane 5 : 3.13 ng
Dilution : 1:1000

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

GTX48820 WB Image

WB analysis of recombinant yeast Ulp1 protein using GTX48820 Ulp1 antibody.
Dilution : 1:1000

Anti-Ulp1 antibody used in Western Blot (WB). GTX48820

GTX48820 WB Image

Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 μg of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TBST, the blot was probed overnight at 4ºC with a 1:1,000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399