GeneTex
United States (US)

Uromucoid antibody

Cat No. GTX29029

Host Sheep
Clonality Polyclonal
Isotype IgG
Application DID, IE, RID
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
DID 10 μl
IE 100 μl
RID Neat-1:5

Note :

DID
10 μl antiserum vs 3 μl Uromucoid 50 mg/L

IE
100 μl antiserum vs 5 μl Uromucoid 50mg/L

RID
10 μl antiserum/cm2 in gel vs 5 μl Uromucoid 50 mg/L Neat - 1:5

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

Gives a single arc when tested by IEP against fresh urine. No arcs are visible when tested by IEP against serum.Identity has been confirmed by double diffusion (Ouchterlony) against uromucoid and a known anti-uromucoid.
PROPERTIES

Form

Liquid

Buffer

Glycine Buffered Saline pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

14.80mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Uromucoid, purified from human urine.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

THGP Antibody , FJHN Antibody , HNFJ Antibody , UMOD Antibody , ADMCKD2 Antibody , HNFJ1 Antibody , THP Antibody , MCKD2 Antibody

Background

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Package List Price ($)
$ 339