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V5 tag antibody

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997
Anti-V5 tag antibody used in Western Blot (WB). GTX117997
Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997
Anti-V5 tag antibody used in Western Blot (WB). GTX117997
Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997
Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997
Anti-V5 tag antibody used in Western Blot (WB). GTX117997
Anti-V5 tag antibody used in ChIP assay (ChIP assay). GTX117997
Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997
Anti-V5 tag antibody used in Western Blot (WB). GTX117997
Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

Cat. No. GTX117997

Reference 21
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all V5 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to V5 tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V5 tag antibody

Research Area

DATA IMAGES

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997

GTX117997 IP Image

V5 tag recombinant protein was immunoprecipitated from HeLa transfected cell lysate by using V5 tag antibody (GTX117997). The precipitated V5 tag recomabinant was detected by GTX117997 diluted at 1:1000. A: Input B: IP by V5 tag antibody

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

GTX117997 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody (GTX117997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

GTX117997 ICC/IF Image

V5 tag antibody detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody (GTX117997) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

GTX117997 WB Image

The data was published in the journal Nat Commun in 2019.PMID: 31171792

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997

GTX117997 IP Image

The data was published in the journal Sci Rep in 2016.PMID: 27216889

Anti-V5 tag antibody used in Immunoprecipitation (IP). GTX117997

GTX117997 IP Image

The data was published in the journal Sci Rep in 2016.PMID: 27216889

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

GTX117997 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

Anti-V5 tag antibody used in ChIP assay (ChIP assay). GTX117997

GTX117997 ChIP assay Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

GTX117997 ICC/IF Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

Anti-V5 tag antibody used in Western Blot (WB). GTX117997

GTX117997 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31681015

Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997

GTX117997 ICC/IF Image

The data was published in the journal PLoS One in 2013. PMID: 23717625

REFERENCE

Application Reference

Veit M et al. Int J Mol Sci 2022; 23 (21) Using Alphafold2 to Predict the Structure of the Gp5/M Dimer of Porcine Respiratory and Reproductive Syndrome Virus.
Application : WB
Yan X et al. Transl Oncol 2022; 24 : 101485 Single nucleotide mutation changes the capability of CCN3 in osteosarcoma cell invasion and differentiation.
Yoann Abel et al. Nucleic Acids Res 2022; 50 (4) : 2172-2189 The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.
Application : WB
Jao TM et al. Cancer Lett 2021; 499 : 290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
Application : WB
Metje-Sprink J et al. Sci Rep 2020; 10 (1) : 12956 Crystal structure of the Rab33B/Atg16L1 effector complex.
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Submit a Reference

REVIEW

V5 tag antibody Cat. No. GTX117997

Rating
( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-V5 tag antibody used in IHC (Paraffin sections) (IHC-P). GTX117997
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : IHC-P
Sample : human cancer injected in mouse tissue
Blocking : 5% BSA in TBST, 25°C, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : 0.3% Triton lysis buffer/Sodium Citrate Buffer
Primary Antibody : 1:100, 4°C, > 12Hr
Anti-V5 tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117997
Date : Anonymous submitted on 24-Oct-2011
Score :
Application Tested : ICC/IF
Sample : 293T cells
Blocking : 3% BSA in PBS, 25°C, 1Hr
Fixation : 2% formaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Triton X-100/PBS
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Veit M et al. Int J Mol Sci 2022; 23 (21) Using Alphafold2 to Predict the Structure of the Gp5/M Dimer of Porcine Respiratory and Reproductive Syndrome Virus.
Application : WB
Yan X et al. Transl Oncol 2022; 24:101485 Single nucleotide mutation changes the capability of CCN3 in osteosarcoma cell invasion and differentiation.
Yoann Abel et al. Nucleic Acids Res 2022; 50 (4):2172-2189 The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.
Application : WB
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
Application : WB
Metje-Sprink J et al. Sci Rep 2020; 10 (1):12956 Crystal structure of the Rab33B/Atg16L1 effector complex.
Hoglin BE et al. Gen Comp Endocrinol 2020; 293:113463 Pharmacological properties of whale shark (Rhincodon typus) melanocortin-2 receptor and melancortin-5 receptor: Interaction with MRAP1 and MRAP2.
Lin ST et al. Front Physiol 2019; 10:1308 Transcription Factor Elf3 Modulates Vasopressin-Induced Aquaporin-2 Gene Expression in Kidney Collecting Duct Cells.
Application : WB
Bernascone I et al. Nat Commun 2019; 10 (1):2481 Sfrp3 modulates stromal-epithelial crosstalk during mammary gland development by regulating Wnt levels.
Application : WB
Wolverton EA et al. Gen Comp Endocrinol 2019; 282:113215 Analyzing the signaling properties of gar (Lepisosteus oculatus) melanocortin receptors: Evaluating interactions with MRAP1 and MRAP2.
Barney E et al. Gen Comp Endocrinol 2019; 272:42-51 Elephant shark melanocortin receptors: Novel interactions with MRAP1 and implication for the HPI axis.
Application : ICC/IF
Chen KR et al. Viruses 2018; 10 (12) Toll-Like Receptor 3 Is Involved in Detection of Enterovirus A71 Infection and Targeted by Viral 2A Protease.
Application : WB
Stephanie Grainger et al. bioRxiv 2018; (Epub) EGFR confers exquisite specificity of Wnt9a-Fzd9b signaling in hematopoietic stem cell development.
Application : WB
Santhakumar D et al. Sci Rep 2018; 8 (1):6794 chicken *-induced protein with tetratricopeptide repeats 5 antagonizes replication of rna viruses.
Application : WB
Timblin GA et al. Mol Cell Biol 2017; (Epub) Dual mechanism of Rag gene repression by c-Myb during pre-B cell proliferation.
Application : WB
Chang KH et al. J Formos Med Assoc 2016; Functional properties of LRRK2 mutations in Taiwanese Parkinson disease.
Application : WB
Shimizu H et al. Sci Rep 2016; 6:26618 Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits.
Application : WB
Lin PH et al. J Biomed Sci 2015; 22 (1):44 N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting.
Application : WB, ICC/IF, IP
Liu YJ et al. FEBS Lett 2015; 589 (4):432-9 Aberrant activation of AMP-activated protein kinase contributes to the abnormal distribution of HuR in amyotrophic lateral sclerosis.
Application : ICC/IF
Wang Z et al. Proc Natl Acad Sci U S A 2014; 111 (1):E89-98 Interplay of mevalonate and Hippo pathways regulates RHAMM transcription via YAP to modulate breast cancer cell motility.
Application : WB
Kinoshita Y et al. Bone 2014; 67C:145-151 Functional analysis of mutant FAM20C in Raine syndrome with FGF23-related hypophosphatemia.
Application : WB
Lei L et al. PLoS One 2013; 8 (5):e64529 Identification of a novel nuclear localization signal sequence in Chlamydia trachomatis-secreted hypothetical protein CT311.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169