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VAMP4 antibody

Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348
Anti-VAMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23348
Anti-VAMP4 antibody used in Western Blot (WB). GTX23348
Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348
Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348

Cat. No. GTX23348

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all VAMP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-3 μg/ml
ICC/IF 2 μg/ml
IHC-P 1:100
FACS 3-5 μg/10⁶ cells
IHC Assay dependent

Note :

ICC/IF
ICC/IF staining with this antibody requires saponin permeabilization.
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat VAMP-4 protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle associated membrane protein 4 , VAMP-4 , VAMP24

Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348

GTX23348 IHC-P Image

IHC-P analysis of human heart tissue using GTX23348 VAMP4 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-VAMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23348

GTX23348 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX23348 VAMP4 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-VAMP4 antibody used in Western Blot (WB). GTX23348

GTX23348 WB Image

WB analysis of acid extracts (40 μg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), SH-SY5Y (Lane 3), Mouse Cerebellum (Lane 4), Caki-1 (Lane 5) and K562 (Lane 6) using GTX23348 VAMP4 antibody.
Dilution : 0.5-3 μg/ml

Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348

GTX23348 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX23348 VAMP4 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-VAMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX23348

GTX23348 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX23348 VAMP4 antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399