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VAMP7 antibody

Anti-VAMP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX17175
Anti-VAMP7 antibody used in Western Blot (WB). GTX17175

Cat. No. GTX17175

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all VAMP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Mouse Lung Tissue Lysate(GTX27933) , Human Lung Tissue Slide

Product Note

At least three isoforms of VAMP7 are known to exist; this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7.The immunogen is located within amino acids 20 - 70 of VAMP7.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887554

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vesicle associated membrane protein 7 , SYBL1 , TI-VAMP , TIVAMP , VAMP-7

Cellular Localization

Lysosome membrane,Endoplasmic reticulum membrane

Background

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-VAMP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX17175

GTX17175 IHC-P Image

IHC-P analysis of human lung tissue using GTX17175 VAMP7 antibody.
Working concentration : 20 μg/ml

Anti-VAMP7 antibody used in Western Blot (WB). GTX17175

GTX17175 WB Image

WB analysis of mouse lung tissue lysate using GTX17175 VAMP7 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

Application Reference

Villeneuve J et al. J Cell Biol 2017; (Epub) Unconventional secretion of FABP4 by endosomes and secretory lysosomes.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399