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VAV3 antibody, Internal

Anti-VAV3 antibody, Internal used in Western Blot (WB). GTX89695
Anti-VAV3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89695

Cat. No. GTX89695

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all VAV3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89695-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SGEQGTLKLPEK, from the internal region of the protein sequence according to NP_006104.4; NP_001073343.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vav guanine nucleotide exchange factor 3

Background

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VAV3 antibody, Internal used in Western Blot (WB). GTX89695

GTX89695 WB Image

WB analysis of human bone marrow lysate using GTX89695 VAV3 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-VAV3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89695

GTX89695 IHC-P Image

IHC-P analysis of human spleen using GTX89695 VAV3 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399