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VAV3 (phospho Tyr173) antibody

Orthogonal
Anti-VAV3 (phospho Tyr173) antibody used in Western Blot (WB). GTX24764

Cat. No. GTX24764

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
50 μl ($399)
See all VAV3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1 μg/ml
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Vav3 that contains tyrosine 173.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vav guanine nucleotide exchange factor 3

Background

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-VAV3 (phospho Tyr173) antibody used in Western Blot (WB). GTX24764

GTX24764 WB Image

Orthogonal

WB (peptide competition) analysis of Cos7 cells over-expressing murine Vav3 were serum starved and left treated (Lane 1) or treated (Lane 2-5) with EGF using GTX24764 VAV3 (phospho Tyr173) antibody prior incubated with the non-phospho immunogen (Lane 3), a generic (pY) peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399